A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER

A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER

Blog Article

While the physical bostik universal primer pro interactions between the Golgi apparatus (Golgi) and lipid droplets (LDs) have been suggested through system-level imaging, the Golgi-LD membrane contact sites (MCSs) remain largely uncharacterized.Here, we show evidence to support the existence of Golgi-LD MCSs in HEK293 cells.We further suggest that vacuolar protein sorting-associated protein 13B (VPS13B) localizes to and promotes the formation of Golgi-LD contacts upon oleic acid (OA) campicon.com stimulation using 3D high-resolution microscopy.Depletion of VPS13B moderately affects the formation of Golgi-LD contacts upon OA treatment in addition to the fragmentation of the Golgi.Although cellular functions of VPS13B-mediated contacts are still elusive, these findings may provide a new insight into related diseases caused by loss-of-function mutations of VPS13B.